Welcome to TRANS-INT

TRANS-INT - New oral nanomedicines: Transporting therapeutic macromolecules across the intestinal barrier

 

This is a large-scale collaborative research project within the 7th European Framework Programme for Research and Technological Development. The TRANS-INT project will run from May 1st, 2012 to April 30th, 2017.

 

In TRANS-INT a clear concept and shared ambition provide the cohesive force. The concept behind TRANS-INT is the rational design of oral nanomedicines based on safety, mechanistic, bioengineering and pharmaceutical technology criteria.

 

The consortium is composed of 16 partners from industry and academia with complementary leading expertise in chemistry, chemical engineering, pharmaceutical technology, molecular physiology, biopharmaceutics, toxicology and drug development.

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Core-shell nanostructures

USC image left

Prof. Alonso's team has developed core-shell nanostructures to confront mucosal barriers (image below). Some of these nanostructures are able to diffuse accross mucus barriers and penetrate into cells (image left)

 

  USC image below

Lipid nanocapsules

new1Prof. Benoit's group has developed new nano-cargos, the lipid Nanocapsules with sizes below the endothelium fenestration ( < 100 nm), that are prepared to encapsulate efficiently lipophilic drugs, offering a pharmaceutical solution for their oral administration

Novel functionalized cyclodextrin nanoparticles

Odriscoll nanoparticlesProfessor O’Driscoll’s group have developed functionalised cyclodextrin-based nanoparticles for siRNA delivery which have achieved gene silencing of TNF-alpha in a murine model of acute colitis. This work, McCarthy et al., has been accepted for publication in the J Cont Release, 2013.

Single multiple Pill - SmPill®

SMPill sigmoid

SmPill® is an innovative drug delivery technology developed at Sigmoid Pharma. It addresses in an integrative manner the issues formulators face when dealing with small molecules and biologics.

Sigmoid is utilising SmPill to create novel therapeutics designed to address unmet clinical needs in a range of diseases as well as enabling oral vaccine and peptide delivery.

Nanoparticles interacting with the mucus

new2Understanding the mechanism of nanoparticle delivery in the gut is key to creating optimised delivery systems. Dr. Schatzlein's group has used confocal microscopy and CARS to study interaction of nanoparticles with the mucus and enterocyte transcytosis. The image shows intestinal villi and enterocytes (green) are in close contact to mucus (blue). The nanoparticles are imaged without fluorescent label using CARS (red).

Nanoparticles in the intestine

NPs in intestineThe fate of biodegradable flourescein- loaded PLG microparticles in microvilli taken from a rat  following intestinal instillations can be observed in the following flourescence microscopy image taken by Prof. Brayden's group. This paper has been cited 133 times (Web of Science).  McClean, S.  et al. (1998). Binding and uptake of biodegradable poly-DL-lactide  micro-  and nanoparticles in intestinal epithelia.  Eur. J. Pharm. Sci. 6, 153-163. 

 

Nanoparticles transport across the intestinal epithelium

across intestine UU

Uppsala Universitiy will start its activity in the second half of 2013.

The picture shows nanoparticle transport across the intestinal epithelium from an earlier project.

Identification of potential biomarker of toxicity using metabolomic approach

Metabolomic CEA

Dr. Mabondzo's group will use a metabolomic approach to build metabolomic maps pointing out the putative dysfuntions occurring during the interactions between epithelial cells and nanocarriers.

Veneto Nanotech-ECSIN activities in nanotoxicology

venetonanotoxicologia

The  main focus of Veneto Nanotech-ECSIN is to study the impact of engineered nanomaterials on human health and environment. In this context, cell biology, molecular biology, biochemistry, as well as fluorescent confocal and electron microscopy techniques are employed to unveil the interaction and effect of nanoparticles exposure to cells.